Application

This Anti-CD44v6 Antibody, exon v6, clone VFF-18 is validated for use in FC, WB, IC, IH(P) for the detection of CD44v6.

Immunohistochemistry on acetone fixed cryostat sections using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedures. Antibody may be used at a concentration of 1 - 2 μg/mL.

Immunohistochemistry on paraffin embedded sections after microwave treatment (1 x 5 min). Antibody may be used at a concentration of 1 - 2 μg/mL.

Immunocytochemistry on cell smears.

Western blot

Flow cytometry: 1:20 dilution

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: exon v6

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in PBS with no preservative.

Specificity

Specific for an epitope encoded by exon v6 on the variant portion of human CD44.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.